Objective To investigated whether exosomes from human umbilical cord-derived MSCconditioned medium (hu-MSC-CM) could increase the survival and function of neonatal porcine islet cell clusters （NICCs）exposed to hypoxia. Methods NICCs were cultured with Hu-MSC-CM and native medium RPMI1640（control）under hypoxic conditions（1% O2 ），with or without exosomes. The effects of exosomes on NICCs viability and function in vitro were examined by FACS and the extracellular Flux assay，respectively. The expression of HIF-1α, PDH2 and VEGF related with hypoxia tolerance genes in NICCs were detected by real-time quantitative PCR. Results Compared with NICCs cultured group with RPMI-1640 medium and Hu-MSC-CM group without exosomes，the survival rate，viability and function were increased in Hu-MSC-CM group with exosomes〔IEQ ： 7 800±210 vs. 5 894±188 4 740±273，P ＜ 0.001 ；β cell viability（%）：77.2±7.0 vs. 68.8±1.8、61.5±5.1， P ＜ 0.05〕. The expression of HIF-1α, PDH2 and VEGF in Hu-MSC-CM group with exosomes was significantlyhigher than the other two groups. Conclusion Hu-MSC-CM could protect NICCs from hypoxia-induced dysfunction, and exosomes played an important role in hypoxic resistance.

目的 探讨来自人类脐带源间充质干细胞条件培养基（human umbilical cord-derived MSCconditioned medium，Hu - MSC-CM）中的外泌体能否增加新生猪胰岛细胞（neonatal porcine islet cell clusters， NICCs）在缺氧环境下的生存和功能。方法 NICCs 在低氧条件下（1% O2）分别用含外泌体的条件培养基、 去除外泌体的条件培养基和常规培养基进行培养。荧光激活细胞分选仪和细胞外通量测定法分别检测外泌 体对细胞活性和功能的影响，实时定量 PCR 检测 NICCs 中缺氧耐受相关基因 HIF-1α、PDH2 及 VEGF 的 表达。结果 与常规培养基组和去除外泌体的条件培养基组相比，含外泌体的条件培养基组培养的 NICCs 其存活率、活性和功能均有所提高〔IEQ ：7 800±210 比 5 894±188、4 740±273，P ＜ 0.001 ；β 细胞存 活率（%）：77.2±7.0 比 68.8±1.8、61.5±5.1，P ＜ 0.05〕。含外泌体的条件培养基组孵育的 NICCs 细胞中 HIF-1α、PDH2 及 VEGF 基因表达均显著高于另外两组。结论 人类脐带源 MSC - 条件培养基可以减轻 NICCs 在缺氧过程中导致的功能障碍，外泌体在诱导低氧抵抗方面发挥重要作用。