Abstract:

Objective To explore the deference expression of genes related with acute rejection（AR）by gene array，in order to provide experimental evidence for improve the level of early diagnosis and the efficacyof renal transplantation. Methods The peripheral blood were collected at the day of operation and kidneypuncture biopsy and were served as the control sample and lab sample，

total RNA were extracted，cDNA probe weresynthesized by reverse transcription，array hybridization were implemented，differentially expressed genes werescreened. Results The study discovered immune gene in peripheral blood lymph cell which was possibly relatedwith AR，there were 20 up-regulated and 11 down-regulated immune associated gene differential expression in

peripheral blood lymph cell. Conclusion There were immune gene with AR may be related in perioheral blood lymph cell and the immune gene whose express-dose appears difference at the AR has been filtered.

摘要：

目的 通过基因芯片技术研究与急性排斥反应（AR）相关的差异表达基因，为提高肾移植术后 AR 的早期诊断水平及肾移植的疗效提供实验依据。方法 以手术当日和移植肾穿刺活检当日收集的外周血作为对照血样和实验血样，提取总 RNA，反转录合成 cDNA，芯片杂交，筛选差异表达基因。结果 发现了外周血淋巴细胞中与 AR 可能相关的免疫基因，其中外周血淋巴细胞免疫相关基因差异表达上调 20 个基因，下调 11 个基因。结论 外周血淋巴细胞中存在与 AR 可能相关的免疫基因，筛选出 AR 时 表达量出现差异的基因。