Abstract:

Objective To study the changes in the level of serum proteins and amino acids in rats performedpartial hepatectomy when portal vein was fully arterialized，and to explore the effect on protein synthesis and amino acid metabolism in the liver by portal vein arterializations（PVA）. Methods The rat model of PVA was established using cuff technique. Twenty of the Sprague-Dawley rats in each group were randomly selected. Rat models in group A were those with portal vein arterialization combining 40% liver resection and left nephrectomy，those in group B were with 40% hepatectomy with left nephrectomy，and those in group C were performed the left nephrectomy alone . On the third and seventh day after operation，the levels of serum amino acid profile were analyzed by automatic amino acid analyzer. Results The success rate of group A was 93.3%（28/30），and that of group B and C were both 100%. Serum amino acids ：Ⅰ：3 days after surgery，Ala，Tyr，Phe，Lys in group A and B compared with group C increasedhighly ；Val，Ile，Leu，Arg，Trp，BCAA/AAA in group A and B compared with group C decreased highly，all of the difference had statistically significant（all P＜0.05）. Comparing with group B，Val in group A decreased highly ； Leu and Lys increased highly，with statistically significant difference（all P＜0.05）；while no statistically significant difference had been found in other parameters（all P＞0.05）. Ⅱ：7 days after surgery，Ile，Leu，Arg，Trp，BCAA/ AAA in group A comparing with group B increased highly，Tyr，Phe decreased highly ；when comparing with group C，Ala，Lys increased highly，and all of the difference was statistically significant（all P＜0.05）. Ala，Tyr，Phe，Lys in group B comparing with group C increased highly, and Ile，Leu，Arg，Trp，BCAA/AAA decreased highlywith statistically significant difference（all P＜0.05）. As far as the changes of other amino acids were concerned, no statistically significant difference had been found between each other group（all P＞0.05）. Conclusions The levels of amino acids recover effectively after portal vein arterializations，and the arterialized group is better than the non-arterialized group. The portal vein arterialization can enhance the accommodation of amino acid in the early period after hepatectomy.

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摘要：

目的 研究当完全门静脉动脉化（PVA）时，大鼠部分肝脏切除（肝切）后血清蛋白质及氨基酸谱水平的变化，来探讨 PVA 对肝脏蛋白质合成及氨基酸代谢的影响。方法 采用袖套法建立大鼠 PVA 动物模型，每组随机选取 SD 大鼠 20 只，A 组为 PVA 联合 40% 肝切 + 左肾切除组，B 组为 40% 肝切 + 左肾切除组，C 组为单纯左肾切除组。术后 3 天和 7 天用全自动氨基酸分析仪检测血清氨基酸谱水平。 结果 A 组手术成功率为 93.3%（28/30），B 组和 C 组手术成功率均为 100%。血清氨基酸谱 ：Ⅰ ：A、B 两 组大鼠于术后第 3 天血清中丙氨酸、络氨酸、苯丙氨酸、赖氨酸水平较 C 组明显升高，缬氨酸、亮氨酸、 异亮氨酸、精氨酸、色氨酸水平及支链氨基酸 / 芳香族氨基酸（BCAA/AAA）比值较 C 组明显降低，差异均 有统计学意义（均 P＜0.05），其中 A 组缬氨酸水平较 B 组明显降低，亮氨酸和赖氨酸水平较 B 组明显升高， 差异均有统计学意义（均 P＜0.05），其余氨基酸水平比较差异均无统计学意义（均 P＞0.05）。Ⅱ ：A 组大 鼠于术后第 7 天血清中异亮氨酸、亮氨酸、精氨酸、色氨酸水平及 BCAA/AAA 比值较 B 组明显升高，络氨酸、 苯丙氨酸水平较 B 组明显降低，丙氨酸、赖氨酸水平较 C 组升高明显，差异均有统计学意义（均 P＜0.05）。 B 组大鼠血清中丙氨酸、络氨酸、苯丙氨酸、赖氨酸水平较 C 组明显升高，异亮氨酸、亮氨酸、精氨酸、 色氨酸水平及 BCAA/AAA 比值较 C 组明显降低，差异均有统计学意义（均 P＜0.05），其余氨基酸水平比较差异均无统计学意义（均 P＞0.05）。结论 PVA 后可有效地恢复血清氨基酸水平，且动脉化组较非动脉化组更能在短期内迅速恢复，对早期血浆氨基酸水平代谢调节有增强作用。

关键词:

大鼠 , 动物模型 , 袖套法 , 门静脉动脉化 , 肝切除 , 氨基酸谱